Features

Rapid and convenient diagnosis: Patient infection status and pathogen load are displayed for up to 96 samples per run.

Generate standard curves: Use spiked samples at known concentrations of target pathogens to generate a standard curve in saliva, blood, urine, or feces.

Record sample traces: Collect real-time LAMP reaction traces from saliva, blood, urine, or feces.

Analyze sample data: Instantly analyze sample traces against a standard curve to identify pathogen presence and concentration in each well.

Export traces for further analysis: All LAMP traces are saved to a run file, which can be accessed on a personal computer (as a .csv file) for in-depth analysis of each sample.

Live monitoring of 96 samples: Fluorescence images from up to 96 samples are displayed live during the run for visual reaction monitoring.

How it works: Bacticount was developed for use with smaRT-LAMP reaction protocols, as described in our JAMA Network Open and EBioMedicine publications. It relies on a nucleic acid detection reaction termed LAMP (Loop-mediated Isothermal Amplification) in a test kit, and is mixed with samples which are monitored by the app. When a pathogen is present, probes bind to its genome, fluorescent dyes in the lab kit are then released and begin to glow. When larger numbers of pathogens are detected, there is faster signal amplification, and fluorescence occurs earlier. The app measures when the fluorescence begins, and interprets pathogen load based on this timing.

Disclaimer: Bacticount is a proof of concept for the scientific community, and has not been approved to diagnose or treat any disease.